W DOC AIRBUS | AMM A320F

Sample Fuel for Microbiological Contamination Analysis

TASK 12-32-28-281-003-A
Sample Fuel for Microbiological Contamination Analysis

WARNING: BE CAREFUL WHEN YOU USE CONSUMABLE MATERIALS. OBEY THE MATERIAL MANUFACTURER'S INSTRUCTIONS AND YOUR LOCAL REGULATIONS.
WARNING: PUT THE SAFETY DEVICES AND THE WARNING NOTICES IN POSITION BEFORE YOU START A TASK ON OR NEAR:
  • THE FLIGHT CONTROLS
  • THE FLIGHT CONTROL SURFACES
  • THE LANDING GEAR AND THE RELATED DOORS
  • COMPONENTS THAT MOVE.
MOVEMENT OF COMPONENTS CAN KILL OR CAUSE INJURY TO PERSONS AND/OR CAN CAUSE DAMAGE TO THE EQUIPMENT.
WARNING: MAKE SURE THAT YOU HAVE THE CORRECT FIRE FIGHTING EQUIPMENT AVAILABLE.
ZONE: 190
ZONE: 500
ZONE: 600
1. Reason for the Job
Refer to the MPD TASK: 281100-04
SAMPLE FUEL FOR MICROBIOLOGICAL CONTAMINATION ANALYSIS
This procedure lets you do a test of the sample locally or you can send it to the laboratory. If it is necessary, you can do a local test and send a sample to the laboratory.
This procedure lets you make a different decision about the procedure that you will use each time you do the procedure. Make your decision about:
  • The environmental conditions at the location of the aircraft
  • The equipment and the services that are available at this location.
It is important that there are no additives in the sample.
Airbus recommends that the fuel in each tank should be more than 10%, which is necessary for the satisfactory operation of the water drain valves. You can refuel as necessary.
Airbus recommends that you give a minimum of 60 minutes of interval between the refuel and the sampling.
Airbus recommends you to do this task together with AMM task 12-32-28-281-001-A.
Airbus recommends that you to do this procedure again in 5 days after you receive the result to confirm the contamination.
2. Job Set-up Information
 A. Fixtures, Tools, Test and Support Equipment
REFERENCE
QTY
DESIGNATION
No specific
1
CONTAINER - CLEAN, APPLICABLE
No specific
AR
CONTAINER 3 L(3/4 USGAL)
No specific
AR
CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS
No specific
AR
CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC
No specific
1
FUEL CONTAMINATION TEST KIT - BIOCIDE
No specific
1
MICROBMONITOR2 TEST KIT ECHA MIC. UK(CAGE CODE OHZY6)
No specific
AR
PROBE - NON METALLIC, FLEXIBLE
No specific
AR
SAFETY BARRIER(S)
No specific
AR
WARNING NOTICE(S)
No specific
AR
WATER - DISTILLED
98A28101000000
1
ADAPTING PIPE - WATER DRAINING FROM FUEL
98A28104000000
1
PURGER - WATER DRAIN
98A28104000002
1
TOOL - PURGING
98D28104000000
1
PIPE ADAPTING-WATER DRAINING
98D28108293000
1
ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK
98D28108293001
1
ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK
98D28108293002
1
ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK
 B. Consumable Materials
REFERENCE
DESIGNATION
(Material No.08BBD1)
Non Aqueous Cleaner-- Isopropyl Alcohol -
 C. Work Zones and Access Panels
ZONE/ACCESS
ZONE DESCRIPTION
190
BELLY FAIR, AIR CONDITIONING & HYD COMPT
500
LEFT WING
600
RIGHT WING
195BB, 196BB
134AR, 153AZ, 197AB, 197GB
 D. Referenced Information
REFERENCE
DESIGNATION
TASK 12-31-28-660-001-A
Cold Weather Maintenance - Fuel
TASK 28-00-00-910-001-A
Fuel Safety Procedures
TASK 28-11-00-600-003-A
Removal of the Microbiological Particles
TASK 28-11-00-600-021-A
Curative Treatment Procedure for Microbiological Contamination - Biobor Method
TASK 28-11-00-600-022-A
Curative Treatment Procedure for Microbiological Contamination - Kathon Method
TASK 32-00-00-481-001-A
Installation of the Safety Devices on the Landing Gears
TASK 52-42-15-000-001-A
Removal of the Access Panel (134AR,154AR)
TASK 52-42-15-400-001-A
Installation of the Access Panel (134AR,154AR)
TASK 53-35-13-000-001-A
Removal of the Belly Fairing Access Panels
TASK 53-35-13-400-001-A
Installation of the Belly Fairing Access Panels
F Maintenance Plan Flowchart - Microbiological Contamination
F Microbiological Contamination Test Kits - Comparison Table
F Fuel Sampling For Analysis ** ON A/C NOT FOR ALL
F Fuel Sampling For Analysis ** ON A/C NOT FOR ALL
F Fuel Sampling For Analysis ** ON A/C NOT FOR ALL
F Fuel Sampling for Analysis ** ON A/C NOT FOR ALL
F Fuel Sampling for Analysis ** ON A/C NOT FOR ALL
3. Job Set-up
Subtask 12-32-28-941-055-B
A. Safety Precautions
WARNING: OBEY THE FUEL SAFETY PROCEDURES.
   (1) As necessary, use the applicable SAFETY BARRIERS, specified by the operator's instructions and your local regulations.
   (2) Make sure that the ground safety locks are installed on the landing gear (Ref. AMM TASK 32-00-00-481-001).
   (3) In the cockpit, on panel 400VU:
  • Make sure that landing-gear control-lever 6GA is in the DOWN position.
  • Put a WARNING NOTICE(S) in position to tell persons not to operate the landing gear.
   (4) Put the WARNING NOTICE(S) in position to tell persons not to operate the landing gear doors.
   (5) Obey the fuel safety procedures when you do work on the fuel system (Ref. AMM TASK 28-00-00-910-001).
   (6) If the temperature of the fuel is less than 0 DEG.C (32.00 DEG.F) and/or the water drain valves are frozen, do one of the two steps that follow:
  • Do the cold-weather maintenance procedure to make the drain valve warm (Ref. AMM TASK 12-31-28-660-001).
  • Remove the fuel samples 60 minutes after the refuel procedure.
Subtask 12-32-28-010-054-B
B. Get Access
   (1) For access to the center tank water drain valves, open the applicable access panels:
  • for water drain valve 94QM, open 195BB
  • for water drain valve 95QM, open 196BB.
Subtask 12-32-28-010-061-B ** ON A/C NOT FOR ALL
C. Get Access (If ACT1 and ACT2 Installed)
   (1) Remove the applicable access panels:
  • for water drain valve 174QM1, remove 197MB and 154CZ
  • for water drain valve 175QM1, remove 197NB and 153BZ
  • for water drain valve 174QM2, remove 154DR
  • for water drain valve 175QM2, remove 153BR.
NOTE: It is necessary to remove panels 197MB and 197NB to gain access to panels 154CZ and 153BZ.
Subtask 12-32-28-010-073-B ** ON A/C NOT FOR ALL
D. Get Access
   (1) Remove the applicable access panels:
Subtask 12-32-28-110-051-A
E. Cleaning
   (1) Mix a solution of 1 part of WATER - DISTILLED to 3-4 parts of Non Aqueous Cleaner-- Isopropyl Alcohol - (Material No.08BBD1) in a CONTAINER - CLEAN, APPLICABLE.
   (2) Clean the applicable drain tool and the CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC with the solution.
NOTE: If you use a CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS, cleaning of the CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS with the solution is not necessary.
   (3) Make sure that the applicable drain tool and the CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC are fully dry before you use them.
Subtask 12-32-28-480-051-A
F. Preparation of the Equipment
   (1) If it is necessary to get access to the high water drain-valves from the ground, attach the:
4. Procedure
Subtask 12-32-28-281-055-B ** ON A/C NOT FOR ALL
A. Sample Fuel for Microbiological Contamination Analysis
If you use a test kit, refer to the kit manufacturers instructions on fuel sampling and storage. Carefully follow the manufacturers instructions to make sure that the test results are accurate. Make sure you use a sample container that is clean and dry before use. An incorrect, dirty sample container can give an unwanted effect to the results.
In the procedure that follows, Airbus recommends that you use Decision C. If you use Decision C, you can make sure that:
  • The results from the local test kit is correct
  • The test kit you used is applicable to your aircraft, its location and the operational conditions.
    In the procedure that follows:
  • You can make one of the decisions A, B or C
  • You can make a different decision each time you do the procedure.
   (1) When you do this procedure, you can make one of these decisions:
     (a) Decision A:
  • Use one of the procedures at paragraph 4.B. thru para 4.D. Each of these give the applicable procedure to use for the applicable test kit you have available.
NOTE: This decision is sufficient when you are sure that the test kit gives accurate results.
     (b) Decision B:
  • Send the samples for an IP385 test at an approved laboratory. Use the test results to do the procedure at para 4.E.
NOTE: You can do this decision when the test kit is not available or when you think it is a better decision for you.
     (c) Decision C:
  • Use an applicable test kit and also send samples for an IP385 test.
NOTE: This makes sure that your test kit results agree with the IP385 Test. You can think of the IP385 test as the benchmark.
   (2) For the wing-tank water drain valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000000 PURGER - WATER DRAIN and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
NOTE: If you do Decision C, you must get two separate samples at each drain. After a kit test, the used samples are considered to have contamination and thus cannot be sent for the IP385 Test.
     (b) Remove the 98A28104000000 PURGER - WATER DRAIN from the drain valve.
   (3) For the center-tank water drain valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000002 TOOL - PURGING and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
     (b) Remove the 98A28104000002 TOOL - PURGING from the drain valve.
   (4) Examine the applicable water drain valve for leakage:
     (a) Make sure that there are no leaks.
     (b) This step is very important at center-tank water-drain-valves 94QM and 95QM, where access is not easy.
   (5) Put the fuel sample for the microbiological test into a CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS or CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC and seal the container:
     (a) If possible, make sure that the fuel sample contains a layer of water and a layer of fuel.
     (b) Do not put additives into the sample to show the water contents.
   (6) Do the sample removal procedure at all of the water drain-valves:
     (a) If tank fuel quantity is less than 10% of the tank total volume, it will be necessary to refuel to at least 10%.
   (7) Identify each sample with a label that shows:
  • The aircraft number
  • The time and date of the sample
  • The fuel tank drain valve the sample came from
  • All the additives you know about.
   (8) Keep the sealed sample containers in a cool protected area:
     (a) After the test samples are collected, microbiological contamination in the sample can multiply or can die. It is recommended that the samples are tested in less than 6 hours and no later than 24 hours. Airbus recommends that the samples are tested in less than 6 hours.
NOTE: Make a selection of one of the microbiological test kits for the complete procedure. Do not compare results between microbiological test kits. They use different methods to give results. If you need help, refer to the manufacturer of the kit.
Subtask 12-32-28-281-055-D ** ON A/C NOT FOR ALL
A. Sample Fuel for Microbiological Contamination Analysis
F Fuel Sampling For Analysis ** ON A/C NOT FOR ALL
If you use a test kit, refer to the kit manufacturers instructions on fuel sampling and storage. Carefully follow the manufacturers instructions to make sure that the test results are accurate. Make sure you use a sample container that is clean and dry before use. An incorrect, dirty sample container can give an unwanted effect to the results.
In the procedure that follows, Airbus recommends that you use Decision C. If you use Decision C, you can make sure that:
  • The results from the local test kit is correct
  • The test kit you used is applicable to your aircraft, its location and the operational conditions.
    In the procedure that follows:
  • You can make one of the decisions A, B or C
  • You can make a different decision each time you do the procedure.
   (1) When you do this procedure, you can make one of these decisions:
     (a) Decision A:
  • Use one of the procedures at paragraph 4.B. thru para 4.D. Each of these give the applicable procedure to use for the applicable test kit you have available.
NOTE: This decision is sufficient when you are sure that the test kit gives accurate results.
     (b) Decision B:
  • Send the samples for an IP385 test at an approved laboratory. Use the test results to do the procedure at para 4.E.
NOTE: You can do this decision when the test kit is not available or when you think it is a better decision for you.
     (c) Decision C:
  • Use an applicable test kit and also send samples for an IP385 test.
NOTE: This makes sure that your test kit results agree with the IP385 Test. You can think of the IP385 test as the benchmark.
   (2) For the wing-tank water drain valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000000 PURGER - WATER DRAIN and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
NOTE: If you do Decision C, you must get two separate samples at each drain. After a kit test, the used samples are considered to have contamination and thus cannot be sent for the IP385 Test.
     (b) Remove the 98A28104000000 PURGER - WATER DRAIN from the drain valve.
   (3) For the center-tank water drain valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000002 TOOL - PURGING and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
     (b) Remove the 98A28104000002 TOOL - PURGING from the drain valve.
   (4) For the ACT water drain valves:
     (a) To get access to the ACT water drain valves, attach the 98D28108293000 ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK or 98D28108293001 ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK as follows:
       1 Put the thrust rod (2) into the sleeve (1).
       2 Install the sleeve (1) in the water drain-valve tube.
       3 Hold the sleeve (1) and turn the nut (3) clockwise against the nut (4) to hold the water drain adaptor in position.
     (b) Put a CONTAINER 3 L(3/4 USGAL) below the applicable water drain valve in the correct position below the thrust rod (2).
     (c) Use a PROBE - NON METALLIC, FLEXIBLE to lift the thrust rod (2) against the water drain valve and drain approximately 1.0 l (0.26 USgal) of fuel.
NOTE: If you do Decision C, you must get two separate samples at each drain. After a kit test, the used samples are considered to have contamination and thus cannot be sent for the IP385 Test.
     (d) Remove the probe to close the water drain valve.
     (e) Hold the sleeve (1) and turn the nut (3) counter-clockwise from the nut (4) to release the water drain adaptor from the water drain valve tube.
     (f) Remove the thrust rod (2) from the sleeve (1).
   (5) Examine the applicable water drain valve for leakage:
     (a) Make sure that there are no leaks.
     (b) This step is very important at center-tank water-drain-valves 94QM and 95QM, where access is not easy.
   (6) Put the fuel sample for the microbiological test into a CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS or CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC and seal the container:
     (a) If possible, make sure that the fuel sample contains a layer of water and a layer of fuel.
     (b) Do not put additives into the sample to show the water contents.
   (7) Do the sample removal procedure at all of the water drain-valves:
     (a) If tank fuel quantity is less than 10% of the tank total volume, it will be necessary to refuel to at least 10%.
   (8) Identify each sample with a label that shows:
  • The aircraft number
  • The time and date of the sample
  • The fuel tank drain valve the sample came from
  • All the additives you know about.
   (9) Keep the sealed sample containers in a cool protected area:
     (a) After the test samples are collected, microbiological contamination in the sample can multiply or can die. It is recommended that the samples are tested in less than 6 hours and no later than 24 hours. Airbus recommends that the samples are tested in less than 6 hours.
NOTE: Make a selection of one of the microbiological test kits for the complete procedure. Do not compare results between microbiological test kits. They use different methods to give results. If you need help, refer to the manufacturer of the kit.
Subtask 12-32-28-281-055-E ** ON A/C NOT FOR ALL
A. Sample Fuel for the Microbiological Contamination Analysis
F Fuel Sampling for Analysis ** ON A/C NOT FOR ALL
If you use a test kit, refer to the kit manufacturer's instructions on fuel sampling and storage. Carefully follow the manufacturer's instructions to make sure that the test results are accurate. Make sure that you use a sample container that is clean and dry. An incorrect, dirty sample container can give an unwanted effect to the results.
In the procedure that follows, Airbus recommend that you use Decision C. If you use Decision C, you can make sure that:
  • The results from the local test kit are correct.
  • The test kit that you used is applicable to your aircraft, its location and the operational conditions.
    In the procedure that follows:
  • You can make one of the decisions A, B or C.
  • You can make a different decision each time you do the procedure.
   (1) When you do this procedure, you can make one of the decisions that follow:
     (a) Decision A:
  • Use one of the procedures at paragraph 4.B. thru paragraph 4.D. Each of these paragraphs gives the applicable procedure to use for the applicable test kit that is available.
NOTE: This decision is sufficient when you are sure that the test kit gives accurate results.
     (b) Decision B:
  • Send the samples for IP385 test at an approved laboratory. Use the test results to do the procedure at paragraph 4.E.
NOTE: You can do this decision when the test kit is not available or when you think it is a better decision for you.
     (c) Decision C:
  • Use an applicable test kit and also send the samples for IP385 test.
NOTE: This makes sure that your test kit results agree with IP385 test.
   (2) For the wing tank water-drain-valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000000 PURGER - WATER DRAIN and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve:
       1 If you do Decision C, you must get two different samples at each drain. After the kit test, there will be contamination in the used samples, and thus it cannot be sent for IP385 test.
     (b) Remove the 98A28104000000 PURGER - WATER DRAIN from the drain valve.
   (3) For the center tank water-drain-valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000002 TOOL - PURGING and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
     (b) Remove the 98A28104000002 TOOL - PURGING from the drain valve.
   (4) For the ACT water drain-valves:
     (a) To get access to the ACT water drain-valves, attach the 98D28108293000 ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK or 98D28108293002 ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK as follows:
       1 Put the thrust rod (2) into the sleeve (1).
       2 Install the sleeve (1) in the water drain-valve tube.
       3 Hold the sleeve (1) and turn the nut (3) clockwise against the nut (4) to hold the water drain adaptor in position.
     (b) Put a CONTAINER 3 L(3/4 USGAL) below the applicable water drain valve in the correct position below the thrust rod (2).
     (c) Use a PROBE - NON METALLIC, FLEXIBLE and lift the thrust rod (2) against the water drain valve and drain approximately 1.0 l (0.26 USgal) of fuel:
       1 If you do Decision C, you must get two different samples at each drain. After the kit test, there will be contamination in the used samples, and thus it cannot be sent for IP385 test.
     (d) Remove the probe to close the water drain valve.
     (e) Hold the sleeve (1) and turn the nut (3) counterclockwise from the nut (4) to release the water drain adaptor from the water drain-valve tube.
     (f) Remove the thrust rod (2) from the sleeve (1).
   (5) Examine the applicable water drain valve for leakage:
     (a) Make sure that there are no leaks.
     (b) This step is very important at center-tank water-drain-valves 94QM and 95QM, where access is not easy.
   (6) Put the fuel sample for the microbiological test into a CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS or CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC and seal the container:
     (a) If possible, make sure that the fuel sample contains a layer of water and a layer of fuel.
     (b) Do not put additives into the sample to show the water contents.
   (7) Do the sample removal procedure at all the water drain valves:
     (a) If tank fuel quantity is less than 10% of the tank total volume, it will be necessary to refuel to at least 10%.
   (8) Identify each sample with a label that shows:
  • The aircraft number
  • The time and date of the sample
  • The fuel tank drain-valve from where the sample came from
  • All the additives you know about.
   (9) Keep the sealed sample containers in a cool protected area:
     (a) After the test samples are collected, microbiological contamination in the sample can multiply or can die. It is recommended that the samples are tested in less than 6 hours and no later than 24 hours. Airbus recommends that the samples are tested in less than 6 hours.
NOTE: Make a selection of one of the microbiological test kits for the complete procedure. Do not compare results between microbiological test kits. They use different methods to give results. If you need help, refer to the manufacturer of the kit.
Subtask 12-32-28-281-055-J ** ON A/C NOT FOR ALL
A. Sample Fuel for Microbiological Contamination Analysis
F Fuel Sampling for Analysis ** ON A/C NOT FOR ALL
If you use a test kit, refer to the kit manufacturers instructions on fuel sampling and storage. Carefully follow the manufacturers instructions to make sure that the test results are accurate. Make sure you use a sample container that is clean and dry before use. An incorrect, dirty sample container can give an unwanted effect to the results.
In the procedure that follows, Airbus recommends that you use Decision C. If you use Decision C, you can make sure that:
  • The results from the local test kit is correct
  • The test kit you used is applicable to your aircraft, its location and the operational conditions.
    In the procedure that follows:
  • You can make one of the decisions A, B or C
  • You can make a different decision each time you do the procedure.
   (1) When you do this procedure, you can make one of these decisions:
     (a) Decision A:
  • Use one of the procedures at paragraph 4.B. thru para 4.D. Each of these give the applicable procedure to use for the applicable test kit you have available.
NOTE: This decision is sufficient when you are sure that the test kit gives accurate results.
     (b) Decision B:
  • Send the samples for an IP385 test at an approved laboratory. Use the test results to do the procedure at para 4.E.
NOTE: You can do this decision when the test kit is not available or when you think it is a better decision for you.
     (c) Decision C:
  • Use an applicable test kit and also send samples for an IP385 test.
NOTE: This makes sure that your test kit results agree with the IP385 Test. You can think of the IP385 test as the benchmark.
   (2) For the wing-tank water drain valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000000 PURGER - WATER DRAIN and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
NOTE: If you do Decision C, you must get two separate samples at each drain. After a kit test, the used samples are considered to have contamination and thus cannot be sent for the IP385 Test.
     (b) Remove the 98A28104000000 PURGER - WATER DRAIN from the drain valve.
   (3) For the center-tank water drain valves:
CAUTION: MAKE SURE THAT THE PLUNGER OF THE TOOL ALIGNS CORRECTLY WITH THE WATER DRAIN VALVE. INCORRECT ALIGNMENT OF THE TOOL CAN CAUSE:
  • THE WATER DRAIN VALVE TO STAY IN THE OPEN POSITION AFTER THE TOOL IS REMOVED
  • A FUEL LEAK.
     (a) Push the center of the applicable water drain valve with the 98A28104000002 TOOL - PURGING and drain approximately 1.0 l (0.26 USgal) of fuel from the water drain valve.
     (b) Remove the 98A28104000002 TOOL - PURGING from the drain valve.
   (4) For the ACT water drain valves:
     (a) To get access to the ACT water drain valves, attach the 98D28108293000 ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK or 98D28108293002 ADAPTER-WATER DRAIN, ADDITIONAL CENTER TANK as follows:
       1 Put the thrust rod (2) into the sleeve (1).
       2 Install the sleeve (1) in the water drain-valve tube.
       3 Hold the sleeve (1) and turn the nut (3) clockwise against the nut (4) to hold the water drain adaptor in position.
     (b) Put a CONTAINER 3 L(3/4 USGAL) below the applicable water drain valve in the correct position below the thrust rod (2).
     (c) Use a PROBE - NON METALLIC, FLEXIBLE to lift the thrust rod (2) against the water drain valve and drain approximately 1.0 l (0.26 USgal) of fuel.
NOTE: If you do Decision C, you must get two separate samples at each drain. After a kit test, the used samples are considered to have contamination and thus cannot be sent for the IP385 Test.
     (d) Remove the probe to close the water drain valve.
     (e) Hold the sleeve (1) and turn the nut (3) counter-clockwise from the nut (4) to release the water drain adaptor from the water drain valve tube.
     (f) Remove the thrust rod (2) from the sleeve (1).
   (5) Examine the applicable water drain valve for leakage:
     (a) Make sure that there are no leaks.
     (b) This step is very important at center-tank water-drain-valves 94QM and 95QM, where access is not easy.
   (6) Put the fuel sample for the microbiological test into a CONTAINER-CLEAN, STERILIZED, TRANSPARENT GLASS or CONTAINER-FUEL RESISTANT, TRANSPARENT PLASTIC and seal the container:
     (a) If possible, make sure that the fuel sample contains a layer of water and a layer of fuel.
     (b) Do not put additives into the sample to show the water contents.
   (7) Do the sample removal procedure at all of the water drain-valves:
     (a) If tank fuel quantity is less than 10% of the tank total volume, it will be necessary to refuel to at least 10%.
   (8) Identify each sample with a label that shows:
  • The aircraft number
  • The time and date of the sample
  • The fuel tank drain valve the sample came from
  • All the additives you know about.
   (9) Keep the sealed sample containers in a cool protected area:
     (a) After the test samples are collected, microbiological contamination in the sample can multiply or can die. It is recommended that the samples are tested in less than 6 hours and no later than 24 hours. Airbus recommends that the samples are tested in less than 6 hours.
NOTE: Make a selection of one of the microbiological test kits for the complete procedure. Do not compare results between microbiological test kits. They use different methods to give results. If you need help, refer to the manufacturer of the kit.
Subtask 12-32-28-281-060-A
B. Sample Analysis - MICROBMONITOR2 Test-Kit Procedure
NOTE: The same type of microbial test kit must be used to complete the procedure. Do not compare the results between the microbial test kits. They use different methods to give results. If you need help, contact the kit manufacturer.
   (1) Use a MICROBMONITOR2 TEST KIT ECHA MIC. UK(CAGE CODE OHZY6) (and the manufacturer's instructions) to make an analysis of each sample.
   (2) Do a check of the results:
     (a) If the test result shows contamination (moderate or heavy), do the test again to make sure that the test results are correct. You must use the same type of test kit for the second test.
     (b) Negligible contamination:
       1 The contamination is negligible if:
  • The water sample content has 1000 CFU/ml (Colony Forming Units) or less
  • The fuel sample content has 4000 CFU/liter or less.
       2 This procedure is completed at this time.
       3 Go to Para. Close-Up.
     (c) Moderate contamination:
       1 The contamination is moderate if:
  • The water sample content has between 1000 and 10000 CFU/ml
  • The fuel sample content has between 4000 and 20000 CFU/liter.
       2 Use the same method and do the sample test again in all the fuel tanks. Do the test between 5 and 10 days after you collect the first sample:
         a If the second fuel sample continues to show moderate or heavy contamination, the table in Para. 5.A will give the analysis of the test result.
       3 This procedure is completed as this time.
       4 Go to Para. Close-Up.
     (d) Heavy contamination:
       1 The contamination is heavy if:
  • The water sample contents has more than 10000 CFU/ml
  • The fuel sample contents has more than 20000 CFU/liter.
       2 Use the same method and do the sample test again in all the fuel tanks. Do the test between 5 and 10 days after you collect the first sample:
         a If the second fuel sample continues to show moderate or heavy contamination, the table in Para. 5.A will give the analysis of the test result.
       3 This procedure is completed as this time.
       4 Go to Para. Close-Up.
Subtask 12-32-28-281-062-A
C. Sample Analysis - HY-LITE Jet-A1 Test-Kit-Procedure
NOTE: The same type of microbial test kit must be used to complete the procedure. Do not compare the results between the microbial test kits. They use different methods to give results. If you need help, contact the kit manufacturer.
NOTE: The manufacturer recommends that you can use only a fuel-resistant transparent plastic-container for the test. A glass container can have an unwanted effect on the test result.
   (1) Use a FUEL CONTAMINATION TEST KIT - BIOCIDE (and the manufacturer's instructions) to make an analysis of each sample.
   (2) Do a check of the results:
     (a) If the test result shows contamination (moderate or heavy), do the test again to make sure that the test results are correct. You must use the same type of test kit for the second test.
     (b) Negligible contamination:
       1 The contamination is negligible if the sample contents has 1000 RLU/liter (Relative Light Units) or less.
       2 This procedure is completed as this time.
       3 Go to Para. Close-Up.
     (c) Moderate contamination:
       1 The contamination is moderate if the sample contents has between 1000 and 5000 RLU/liter.
       2 Use the same method and do the sample test again in all the fuel tanks. Do the test between 5 and 10 days after you collect the first sample:
         a If the second fuel sample continues to show moderate or heavy contamination, the table in Para. 5.A will give the analysis of the test result.
       3 This procedure is completed as this time.
       4 Go to Para. Close-Up.
     (d) Heavy contamination:
       1 The contamination is heavy if the sample contents has more than 5000 RLU/liter.
       2 Use the same method and do the sample test again in all the fuel tanks. Do the test between 5 and 10 days after you collect the first sample:
         a If the second fuel sample continues to show moderate or heavy contamination, the table in Para. 5.A will give the analysis of the test result.
       3 This procedure is completed as this time.
       4 Go to Para. Close-Up.
Subtask 12-32-28-281-063-A
D. Sample Analysis - With Results from Laboratory IP385 Test.
NOTE: The same method of microbial test must be used to complete the procedure. Do not compare the results between the microbial test kits. They use different methods to give results. If you need help, contact the kit manufacturer.
   (1) Do a check of the results:
     (a) If the test result shows contamination (moderate or heavy), do the test again to make sure that the test results are correct. You must use the same test method for the second test.
     (b) Negligible contamination:
       1 The contamination is negligible if:
  • The water sample contents has 1000 CFU/ml (Colony Forming Units) or less
  • The fuel sample contents has 4000 CFU/liter or less.
       2 This procedure is completed as this time.
       3 Go to Para. Close-Up.
     (c) Moderate contamination:
       1 The contamination is moderate if:
  • The water sample contents has between 1000 and 10000 CFU/ml
  • The fuel sample contents has between 4000 and 20000 CFU/liter.
       2 Use the same method and do the sample test again in all the fuel tanks. Do the test between 5 and 10 days after you collect the first sample:
         a If the second fuel sample continues to show moderate or heavy contamination, the table in Para. 5.A will give the analysis of the test result.
       3 This procedure is completed as this time.
       4 Go to Para. Close-Up.
     (d) Heavy contamination:
       1 The contamination is heavy if:
  • The water sample contents has more than 10000 CFU/ml
  • The fuel sample contents has more than 20000 CFU/liter.
       2 Use the same method and do the sample test again in all the fuel tanks. Do the test between 5 and 10 days after you collect the first sample:
         a If the second fuel sample continues to show moderate or heavy contamination, the table in Para. 5.A will give the analysis of the test result.
       3 This procedure is completed as this time.
       4 Go to Para. Close-Up.
5. Close-up
Subtask 12-32-28-281-076-A ** ON A/C NOT FOR ALL
A. Analysis of the Results
   (1) Do the analysis of the results of the sample analysis as follows:
First Test
Second Test
Result
Negligible
Not necessary
Negligible
Moderate
Negligible
Negligible
Moderate
Moderate
Moderate
Moderate
Heavy
Heavy
Heavy
Negligible
Moderate
Heavy
Moderate
Moderate
Heavy
Heavy
Heavy
   (2) If the result of the first microbiological contamination test is negligible, no curative treatment is necessary. You can go back to operation or do the parking/storage procedures as applicable.
   (3) If you use Biocide, do the steps that follow:
     (a) Do a check for biocide that was used in last 30 refuellings. To avoid the interaction of different biocides, you must use the same biocide that is used in last 30 refuellings (prevents the formation of precipitates and there is a risk of engine failure).
     (b) If the results of the microbiological contamination tests are moderate, do the microbiological treatment with Biocide in less than 10 days (Ref. AMM TASK 28-11-00-600-021) or (Ref. AMM TASK 28-11-00-600-022).
     (c) If the results of the microbiological contamination tests are heavy, in less than 10 days:
       1 Do the manual cleaning of the affected tanks (Ref. AMM TASK 28-11-00-600-003).
       2 Do the microbiological treatment with the Biocide on all the tanks (including the affected tank) (Ref. AMM TASK 28-11-00-600-021) or (Ref. AMM TASK 28-11-00-600-022).
   (4) If Biocide is not used and if the results of the microbiological contamination tests are moderate or heavy, do the manual cleaning process in 10 days (Ref. AMM TASK 28-11-00-600-003).
   (5) If you return the aircraft from parking or storage and the result is heavy, the tanks must be decontaminated before return to service. You can ignore this step if the aircraft is in operation.
Subtask 12-32-28-281-076-B ** ON A/C NOT FOR ALL
A. Analysis of the Results
   (1) Do the analysis of the results of the sample analysis as follows:
First Test
Second Test
Result
Negligible
Not necessary
Negligible
Moderate
Negligible
Negligible
Moderate
Moderate
Moderate
Moderate
Heavy
Heavy
Heavy
Negligible
Moderate
Heavy
Moderate
Moderate
Heavy
Heavy
Heavy
   (2) If the result of the first microbiological contamination test is negligible, no curative treatment is necessary. You can go back to operation or do the parking/storage procedures as applicable.
   (3) If you use Biocide, do the steps that follow:
     (a) Do a check for biocide that was used in last 30 refuellings. To avoid the interaction of different biocides, you must use the same biocide that is used in last 30 refuellings (prevents the formation of precipitates and there is a risk of engine failure).
     (b) If the results of the microbiological contamination tests are moderate, do the microbiological treatment with Biocide in less than 10 days (Ref. AMM TASK 28-11-00-600-021).
     (c) If the results of the microbiological contamination tests are heavy, in less than 10 days:
       1 Do the manual cleaning of the affected tanks (Ref. AMM TASK 28-11-00-600-003).
       2 Do the microbiological treatment with the Biocide on all the tanks (including the affected tank) (Ref. AMM TASK 28-11-00-600-021).
   (4) If Biocide is not used and if the results of the microbiological contamination tests are moderate or heavy, do the manual cleaning process in 10 days (Ref. AMM TASK 28-11-00-600-003).
   (5) If you return the aircraft from parking or storage and the result is heavy, the tanks must be decontaminated before return to service. You can ignore this step if the aircraft is in operation.
Subtask 12-32-28-410-054-B
B. Close Access
   (1) Make sure that the work area is clean and clear of tools and other items.
   (2) Close the applicable access panels:
  • for water drain valve 94QM, close 195BB
  • for water drain valve 95QM, close 196BB.
Subtask 12-32-28-410-060-B ** ON A/C NOT FOR ALL
C. Close Access (ACT1 and ACT2 Installed)
   (1) Make sure that the work area is clean and clear of tools and other items.
   (2) Install the applicable access panels:
  • for the water drain valve 174QM1, install 154CZ and 197MB
  • for the water drain valve 175QM1, install 153BZ and 197NB
  • for the water drain valve 174QM2, install 154DR
  • for the water drain valve 175QM2, install 153BR.
Subtask 12-32-28-410-070-B ** ON A/C NOT FOR ALL
D. Close Access
   (1) Make sure that the work area is clean and clear of tools and other items.
   (2) Install the removed access panels:
Subtask 12-32-28-942-054-A
E. Removal of Equipment
   (1) Make sure that the work area is clean and clear of tools and other items.
   (2) Remove the safety barriers.
   (3) Remove the ground support and maintenance equipment, the special and standard tools and all other items.
[Rev.10 from 2021] 2026.04.01 04:23:01 UTC
"Aviabrain" 2025